Optimalisasi Dosis Inokulum dan Lama Waktu Fermentasi pada Pembuatan Bioetanol dari Ampas Tebu (Saccharum officinarum L.)

Abstract

The purpose of this research was to determine the optimal conditions of the inoculum dose and the length of time of fermentation of the volume of bioethanol produced from sugarcane bagasse. Bagasse is a by-product of milking sugar cane liquid. Sugarcane bagasse includes lignocellulosic biomass, so it is possible to be utilized as an alternative energy source such as bioethanol. This research method is experimental with Randomized Block Design (RBD). Bagasse is sorted, then bagasse is dried in the sun to dry ( 3 days) and grinded using a blender to produce flour.Then fermented by adding a number of Saccharomyces cereviseae inoculum to the fermentation medium as much 0%, 12%, 14% and 16% with fermentation time during 24, 48, 72, 96 and 120 hours with the addition of urea 0.05% of the mass to be fermented. The fermentation results are purified through a distillation process at 75 derajat C. The results showed that the optimum conditions of inoculum dose and fermentation time was 72 hours with 14% inoculum dose. This means that the length of time of fermentation and the dose of inoculum used does affect the volume of bioethanol produced.