Uji Aktivitas Antimikroba Daun Pandan Wangi (Pandanus amaryllifolius Roxb) terhadap Escherichia coli, Shigella dysentriae dan Candida albicans

Abstract

The purpose of this research was to determine chemical compounds in crude extract of pandanus fragrant leaves and the ability of this compound to slow down the growth of Escherichia coli, Shigella dysentryae and Candida albicans. The method that use for the extraction of pandanus fragrant leaf is a maceration method with n-hexane solvent and ethyl acetat. Experiment activity antimicrobial of pandanus fragrant leaves towards microbial pathogen use diffusion method by Kirby Bauer with variation treatment 25%, 50%, 75% and 100% with the antibiotic antibiotics too look whether pandan leaves extract can be used as an alternative medicine antimicrobial is Chloramphenicol and Nystatin. The result of research showed that pandanus fragrant leaves extract contains secondary metabolit namely flavonoids, alkaloids, triterpenoids and tanin with the most suitable solvent is ethyl acetate. Pandanus fragrant leaves have antimicrobial activity that concern Escherichia coli, Shigella dysentryae and Candida albicans with the largest clear transparan on Escherichia coli in concentrate 100% in the amunt 12,1 mm, on Shigella dysentryae in concentrate 100% in the amunt 12,3 mm and Candida albicans in concentrate 75% in the amunt 21,4 mm.