Uji Bioaktivitas Antimikroba Ekstrak Kasar Batang dan Daun Bangun-bangun (Coleus amboinicus Lour) Terhadap Bakteri Escherichia coli

Abstract

Bangun-bangun (Coleus amboinicus Lour) is one of traditional medicinal plants used to treat certain diseases or be used as an antibacterial. The purpose of this study was to determine the chemical compounds contained in the crude extract of bangun-bangun stems and leaves and to observe the antimicrobial activity of crude extract of bangun-bangun stems and leaves. This research is an experimental study with Completely Rendomized Design (CRD). Samples were extracted by maceration method using ethyl acetate and n- hexane solvent. Antimicrobial effect of the extracts were tested by agar diffusion method with 5%, 10%, 15%, 20%, and 25% concentration. Ciprofloxacin and dimethyl sulfoxide (DMSO) were used as positive and negative control. Observation parameter is the formation of inhibition zone at each extract concentration. The results showed that the bangun-bangun contains secondary metabolite compounds namely flavonoids, tannins, saponins, alkaloids, and triterpenoids. The average of stems inhibition zone diameter were 28.27; 33.46; 35.67; 37.12; 38.46 mm and leaves inhibition zone diameter were 38.58; 39.94; 42.12; 46.79; 47.75 mm. Inhibition zone diameter positive and negative control were 61.08 mm and 18.16 mm