Uji Bioaktivitas Antimikroba Ekstrak Kasar Daun dan Buah Belimbing Wuluh (Averrhoa Bilimbi Linn) Terhadap Bakteri Escherichia coli

Abstract

Belimbing wuluh (Averrhoa bilimbi Linn) is one of traditional medicinal plants used to treat certain diseases or be used as an antibacteria. The purpose of this study was to determine the chemical compounds contained in the crude extract of belimbing wuluh leaves and fruit and to observe the antimicrobial activity of crude extract of belimbing wuluh leaves and fruit. This research is an experimental study with Completely Rendomized Design (CRD). Samples were extracted by maceration method using ethyl acetate and n-hexane solvent. Antimicrobial effect of the extracts were tested by agar diffusion method with 5%, 10%, 15%, 20%, and 25% concentration. Ciprofloxacin and dimethyl sulfoxide (DMSO) were used as positive and negative control. Observation parameter is the formation of inhibition zone at each extract concentration. The results showed that the belimbing wuluh contains secondary metabolite compounds namely flavonoids, tannins, saponins, alkaloids, and triterpenoids. The average of leaf inhibition zone diameter were 23.06; 23.79; 33.16; 38.79; 45.04 mm and fruit inhibition zone diameter were 33.68; 40.56; 45.04; 48.35; 60.04 mm. Inhibition zone diameter positive and negative control were 64.41 mm and 18.16 mm