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https://repositori.uma.ac.id/handle/123456789/29433| Title: | Efektivitas Konsentrasi Biotin Dan Benzyl Amino Purin (Bap) Dalam Media Kultur Terhadap Pertumbuhan Planlet Kentang Lokal Sumatera Utara Secara In Vitro |
| Other Titles: | The Effectiveness of Biotin and Benzyl Amino Purine (BAP) Concentrations in Culture Media on the In Vitro Growth of Local Potato Plantlets from North Sumatra |
| Authors: | Hulu, Lilis Karlin |
| metadata.dc.contributor.advisor: | Riyanto |
| Keywords: | Consentration;Potato;Biotin;Benzyl Amino Purin;Media;Konsentrasi;Kentang |
| Issue Date: | Sep-2025 |
| Publisher: | Universitas Medan Area |
| Series/Report no.: | NPM;218700019 |
| Abstract: | Kentang merupakan tanaman umbi-umbian dan termasuk famili Solanaceae. Kentang mengandung karbohidrat, protein, mineral, dan vitamin serta memiliki manfaat untuk dikonsumsi sehar-hari maupun diolah menjadi produk oleh industri makanan. Rendahnya produksi kentang di Sumatera Utara dipengaruhi oleh perbanyakan dan distribusi benih berkualitas yang tidak konsisten dan benih yang cenderung lebih mahal daripada kentang biasa. Untuk meningktkan produksi kentang dapat ditingkatkan melalui teknik kultur jaringan yang merupakan upaya yang cepat dan relatif singkat untuk memperbanyak bibit kentang dengan kualitas tinggi dan bebas dari penyakit. Tujuan dilakukan penelitian ini untuk mengetahui konsentrasi dan kombinasi terbaik dari pemberian Biotin dan BAP terhadap pertumbuhan planlet kentang lokal Sumatera Utara. Penelitian ini menggunakan RALF dengan 2 faktor. Faktor pertama adalah konsentrasi Biotin 0,5 mg/l, 1 mg/l, 1,5 mg/l, 2 mg/l dan faktor kedua konsentrasi BAP (Benzyl Amino Purin) 0,5 mg/l, 1 mg/l, 1,5 mg/l, 2 mg/l. Hasil penelitian menunjukkan bahwa kombinasi Biotin dan BAP memberikan pengaruh nyata terhadap pertumbuhan planlet pada parameter bobot planlet setelah 8 minggu setelah tanam dengan kombinasi terbaik yaitu Biotin 2 mg/l + BAP 0,5 mg/l, Biotin 2 mg/l + BAP 0 mg/l dan Biotin 2 mg/l + BAP 1 mg/l. Perlakuan dengan BAP saja tidak efektif terhadap pertumbuhan planlet kentang. Perlakuan Biotin saja efektif terhadap pertumbuhan planlet kentang dengan konsentrasi terbaik 2 mg/l. Potatoes are tuberous plants belonging to the Solanaceae family. Potatoes contain carbohydrates, proteins, minerals, and vitamins, and are beneficial for daily consumption or for processing into food products. Low potato production in North Sumatra is influenced by inconsistent propagation and distribution of quality seeds, which tend to be more expensive than regular potatoes. Potato production can be increased through tissue culture techniques, which are a quick and relatively short process for propagating high-quality, disease-free potato seedlings. The purpose of this study was to determine the optimal concentration and combination of Biotin and BAP for the growth of local North Sumatran potato plantlets. This study used RALF with two factors. The first factor was the concentration of Biotin at 0.5 mg/l, 1 mg/l, 1.5 mg/l, and 2 mg/l, and the second factor was the concentration of BAP (Benzyl Amino Purin) at 0.5 mg/l, 1 mg/l, 1.5 mg/l, and 2 mg/l. The results showed that the combination of Biotin and BAP had a significant effect on the growth of planlets in terms of planlet weight after 8 weeks of planting, with the best combinations being Biotin 2 mg/l + BAP 0.5 mg/l, Biotin 2 mg/l + BAP 0 mg/l, and Biotin 2 mg/l + BAP 1 mg/l. Treatment with BAP alone was ineffective for potato planlet growth. Treatment with Biotin alone was effective for potato planlet growth, with the optimal concentration being 2 mg/l. |
| Description: | 61 Halaman |
| URI: | https://repositori.uma.ac.id/handle/123456789/29433 |
| Appears in Collections: | SP - Biology |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 218700019 - Lilis Karlin Hulu - Chapter IV.pdf Restricted Access | Chapter IV | 2.02 MB | Adobe PDF | View/Open Request a copy |
| 218700019 - Lilis Karlin Hulu - Fulltext.pdf | Cover, Abstract, Chapter I, II, III, V, Bibliography | 2.52 MB | Adobe PDF | View/Open |
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