Uji Efektivitas Ekstrak Etil Asetat Rimpang Bangle (Zingiber purpureum Roxb) dalam Menghambat Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli

Abstract

Bangle (Zingiber purpureum) consist of active compounds such as essential oils, terpenoids, flavonoids, tannins, and alkaloids that have antimicrobial activity. This research aimed to find out the ability of ethyl acetate extract of rhizome bangle in inhibiting the growth of Staphylococcus aureus and Escherichia coli. The extraction method used is maceration using ethyl acetate as a solvent. Antibacterial test was performed using disc diffusion method to see the existence of clear zone around the disc in various bangle extract concentration (0%, 20%, 40%, 60%, and 80%). The data obtained were statistically analized using a Complete Randomized Design. Data analysis showed that Escerichia coli and Staphylococcus aureus growth were significantly (P<0,01) inhibited by various concentration of bangle extract. 80% of extract concentration has the greatest effect to those bacteria as shown by diameter of inhibition zone. The highest concentration of extract, the more effective to inhibit bacteria growth.